目的 分析不同产地玄参中化学成分的差异。方法 采用超快速液相-串联四极杆飞行时间高分辨质谱(UFLC-Triple TOF-MS/MS)技术对不同产地12批样品进行测定,通过多级串联质谱分析,对其质谱数据进行峰匹配、峰对齐、滤噪处理等进行特征峰提取;用主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)进行数据处理;通过一级质谱精确质荷比和二级质谱碎片信息,结合软件数据库搜索及相关文献进行成分鉴别。结果 不同产地玄参样品间的化学组成得到明显区分;初步筛选并鉴定出不同产地玄参间36种差异显著的化学成分,且11种共有差异化学成分呈现不同的变化规律。结论 为揭示生态环境对玄参代谢物合成积累的影响规律提供基础资料。
Abstract
OBJECTIVE To analyze the difference of chemical constituents in Scrophulariae Radix from different areas by UFLC-Triple TOF-MS/MS. METHODS Through analysis of the multistage tandem mass spectra, the characteristic peaks were extracted with mass spectrometry data peak matching, peak alignment, and noise filtering. Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used for data processing. The constituents were identified according to MS accurate mass and MS/MS spectrometry fragmentation information, combined with the software of database search and literature. RESULTS The chemical constituents in Scrophulariae Radix from different areas were clearly distinguished. Thirty-six chemical constituents were indentified as differential compounds. Among them, there were 11 kinds of common differential chemical constituents, i.e., caffeic acid, aucubin, 5-hydroxymethylfurfural, harpagide, 6-O-methylcatapol, verbascoside, p-coumaric acid, ningpogenin, 6″-O-feruloylharpagide, sibirioside A, and harpagoside. CONCLUSION This study provides experimental data to reveal the influence of ecological environment and genetic variation on metabolite biosynthesis of Scrophulariae Radix.
关键词
玄参 /
超快速液相色谱-串联四极杆飞行时间高分辨质谱法 /
不同产地 /
植物代谢组学 /
环烯醚萜
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Key words
Scrophulariae Radix /
UFLC-Triple TOF-MS/MS /
different area /
plant metabolomics /
iridoid
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中图分类号：
R284
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参考文献
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脚注
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基金
徐州市科技计划项目资助(KC16SG266);
南通市科技计划项目(JCZ18105)
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